Did treatment clear the trap?
Compare longitudinal burden, event count, size and morphology to identify residual intact structures.
BURDENSIZEMORPHOLOGY
VOLUMETRIC EXTRACELLULAR DNA-TRAP ANALYSIS
See the structure
behind the signal.
Nebulum preserves intact extracellular DNA traps from whole blood and resolves their architecture, molecular localization and cellular interactions in three dimensions.
PLATFORM VISUALIZATION · EXTRACELLULAR DNA-TRAP VOLUMEWHOLE-BLOOD PRESERVATIONHIGH-DENSITY 3D CAPTUREMULTIPLEX PHENOTYPINGOBJECT-LEVEL QUANTIFICATION
01 / VOLUMETRIC BIOLOGY
ONE EVENT · EVERY SCALE OF DISCOVERYFrom one optical plane
From one optical plane
to the complete capture.
Serial optical sections reconstruct an intact event before the view expands into the complete matrix—connecting local biology with population-scale context.
TARGET-ASSOCIATED SIGNAL
CELLULAR CONTEXT
01 · OPTICAL SECTION
Begin at a defined depth
A single optical plane isolates local structure and segmented nuclear morphology within the captured event.
BIOLOGICAL QUESTIONS · BUILT INTO THE ASSAYFrom a captured structure
From a captured structure
to a development decision.
Configure the marker panel and collection timepoints around the mechanism your program needs to resolve.
What changes when immune cells encounter it?
Map enclosed, contacting and adjacent cells together with study-selected identity and state markers.
IDENTITYCONTACTSTATE
Where is immune suppression localized?
Localize PD-L1 to trap structures and phenotype interacting cells with markers such as PD-1, CTLA-4 or TIM-3.
PD-L1 · TRAPPD-1 · CELLCTLA-4 · CELL
02 / THE PLATFORM
Preserve the structure.
Interrogate the biology.
One coordinated workflow converts a whole-blood specimen into a quantitative, three-dimensional structural endpoint.
01Stabilize the biological moment.
Whole blood is preserved at collection so fragile extracellular DNA structures reach analysis with minimal ex vivo distortion.
OUTPUT · Stabilized whole blood
02Concentrate rare structures.
The retained fraction is assembled into a thin, optically accessible matrix and labeled with a study-specific marker panel.
OUTPUT · Target-labeled 3D specimen
03Capture the complete volume.
Light-sheet or confocal acquisition resolves intact events plane by plane throughout the three-dimensional specimen.
OUTPUT · Volumetric fluorescence dataset04Turn structure into an endpoint.
Object-level analysis reports burden, morphology, molecular localization and cell–trap relationships.
OUTPUT · Structural phenotype profileONE SPECIMENPRESERVE → COMPACT → IMAGE → QUANTIFYONE STRUCTURAL DATASET
03 / TRANSLATIONAL SIGNALS
See what clears.
See what remains.
MATCHED HUMAN OBSERVATIONS
Treatment-linked structural change, resolved directly in circulation.
01 / LONGITUDINAL RESPONSESTAGE I CRC · MATCHED CASE
The tumor was removed.
Circulating trap burden fell toward the healthy range.
In one exploratory longitudinal case, abundant large extracellular DNA-trap structures before resection were replaced by sparse residual fragments two weeks later.
DISPLAY-OPTIMIZED PSEUDOCOLOR
DISPLAY-OPTIMIZED PSEUDOCOLOROBSERVED CHANGE≈10×LOWER
RELATIVE TRAP BURDENHealthy reference = 1.0
10.0PRE-RESECTION
≈1.02-WEEK FOLLOW-UP
WHY PRESERVE STRUCTURE?A difference emerged in imaging—not in plasma citH3.STRUCTURE-BASED IMAGINGCRC–healthy separation***VERSUSPLASMA citH3No significant separationns+
EXPLORATORY PILOT COMPARISON
Structure resolved a biological difference that the soluble readout did not.
Intact-event imaging and plasma citH3 measure different biological material. This comparison motivates prospective validation; it is not a formal claim of analytical or clinical sensitivity.
THE STUDY THIS ENABLESCan longitudinal structural burden provide a treatment-responsive endpoint across a prospective colorectal cancer cohort?
Single exploratory longitudinal case · Healthy-normalized legacy analysis · Research use only · Prospective validation required
02 / RESIDUAL BURDENSLE · MATCHED TREATMENT SERIES
Small fragments receded.
Large traps remained.
In one exploratory SLE case, the circulating pattern changed after immunosuppressant therapy—but large intact extracellular DNA traps were still visible.
DISPLAY PSEUDOCOLOR
DISPLAY PSEUDOCOLORRESIDUAL PHENOTYPELARGETRAPS PERSIST
WHAT THIS CASE SHOWSImmunosuppression changed the pattern without eliminating every large circulating structure.
THE NEXT DRUG QUESTIONCan a NET-directed therapy clear the residual structural burden?
Single exploratory case · Representative matched fields · Research use only · No causal or therapeutic inference · Prospective validation required
FROM OBSERVATION TO ENDPOINT
Design the study Test whether the structure responds.
Representative exploratory observations · Research use only · Prospective cohort validation required
SELECTED SCIENTIFIC CONTEXTWhy intact trap biology may matter to therapeutic response+
04 / BUILD THE NEXT STUDY
From an observed signal
to a prospectively tested endpoint.
A focused pilot aligns the biological hypothesis, collection plan and structural analysis with the decision your development program needs to make.
Define the hypothesis
Indication, therapy, cohort, timepoints and the structural signal to test.
Design the measurement
Preservation workflow, marker panel, imaging strategy and quantitative readouts.
Deliver the evidence
Registered 3D images, object-level data and a study-level interpretation.
START WITH A FOCUSED PILOTHave a signal
Have a signal
worth testing?
Bring the samples and therapeutic context. Nebulum will build the measurement strategy around the hypothesis.
YOU PROVIDESamples · treatment context · available outcomes
→NEBULUM DELIVERSPreservation · 3D measurement · structured report
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